Whole-Genome Sequence and Comparative Analysis of Trichoderma asperellum ND-1 Reveal Its Unique Enzymatic System for Efficient Biomass Degradation
نویسندگان
چکیده
The lignocellulosic enzymes of Trichoderma asperellum have been intensely investigated toward efficient conversion biomass into high-value chemicals/industrial products. However, lack genome data is a remarkable hurdle for hydrolase systems studies. secretory newly isolated T. ND-1 during lignocellulose degradation are currently poorly known. Herein, high-quality genomic sequence ND-1, obtained by both Illumina HiSeq 2000 sequencing platforms and PacBio single-molecule real-time, has an assembly size 35.75 Mb comprising 10,541 predicted genes. Secretome analysis showed that 895 proteins were detected, with 211 associated carbohydrate-active (CAZymes) responsible hydrolysis. Additionally, atroviride IMI 206040, virens Gv-298 shared 801 orthologues not identified in reesei QM6a, indicating may play critical roles biological-control. In-depth suggested that, compared the encoded unique enzymatic system, especially hemicellulases chitinases. Moreover, after comparative lignocellulase activities other fungi, we found displayed higher (particularly xylanases) comparable cellulases activities. Our analysis, combined whole-genome information, offers platform designing advanced strains industrial utilizations, such as bioenergy production.
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ژورنال
عنوان ژورنال: Catalysts
سال: 2022
ISSN: ['2073-4344']
DOI: https://doi.org/10.3390/catal12040437